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Literature summary extracted from
- Purification and properties of a glycerophosphocholine phosphodiesterase from bovine brain myelin (1994), Neurochem. Res., 19, 43-48.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.4.2 | 0.22 | - |
p-nitrophenylphosphocholine | - |
Bos taurus |  |
| 3.1.4.2 | 0.23 | - |
glycerophosphocholine | - |
Bos taurus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.2 | 14000 | - |
SDS-PAGE and native PAGE analysis | Bos taurus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.2 | sn-glycero 3-phosphorylcholine + H2O | Bos taurus | cellular phosphatidylcholine catabolic pathway | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.4.2 | Bos taurus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.4.2 | 80-99fold purification | Bos taurus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.4.2 | brain | - |
Bos taurus | - |
| 3.1.4.2 | myelin membrane | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.2 | 0.096 | - |
for glycero 3-phosphocholine hydrolysis | Bos taurus |
| 3.1.4.2 | 0.598 | - |
for p-nitrophenylphosphocholine hydrolysis | Bos taurus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.2 | p-nitrophenylphosphocholine + H2O | - |
Bos taurus | p-nitrophenol + phosphocholine | single enzyme utilizing both glycerophosphocholine and p-nitrophenylphosphocholine as substrates | ? | |
| 3.1.4.2 | sn-glycero 3-phosphorylcholine + H2O | - |
Bos taurus | sn-glycerol 3-phosphate + choline | - |
? | |
| 3.1.4.2 | sn-glycero 3-phosphorylcholine + H2O | cellular phosphatidylcholine catabolic pathway | Bos taurus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.4.2 | monomer | 1* 14000, SDS-PAGE | Bos taurus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.2 | 10 | - |
for glycerophosphorylcholine hydrolysis | Bos taurus |
| 3.1.4.2 | 11 | - |
for p-nitrophenylphosphocholine hydrolysis | Bos taurus |
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Release 2023.1 (January 2023)
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